Immunofluorescent demonstration of double-stranded RNA and virus antigen in RNA virus-infected cells

The indirect immunofluorescence procedure has been used for demonstration of double-stranded RNA in cells infected with reovirus, poliomyelitis, and tick-borne encephalitis (TBE) viruses. Rabbit sera against poly(A)-poly(U) and poly(I)-poly(C) react specifically with double-stranded RNA. Double-stra...

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Bibliographic Details
Published inVirology (New York, N.Y.) Vol. 62; no. 1; pp. 276 - 279
Main Authors Gavrilovskaya, I.N., Lavrova, I.K., Voroshilova, M.K., Chumakov, M.P., Poverenny, A.M., Podgorodnichenko, V.K.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.11.1974
Subjects
Animals
Antigens, Viral - isolation & purification
Cell Line
Cytoplasm - immunology
Encephalitis Viruses, Tick-Borne - growth & development
Encephalitis Viruses, Tick-Borne - immunology
Encephalitis Viruses, Tick-Borne - metabolism
Fluorescent Antibody Technique
Haplorhini
HeLa Cells
Humans
Immune Sera
Kidney
Poliovirus - growth & development
Poliovirus - immunology
Poliovirus - metabolism
Poly A-U - immunology
Poly I-C - immunology
Rabbits - immunology
Reoviridae - growth & development
Reoviridae - immunology
Reoviridae - metabolism
RNA, Viral - biosynthesis
RNA, Viral - isolation & purification
Swine
Viral Proteins - biosynthesis
Virus Replication
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Summary:The indirect immunofluorescence procedure has been used for demonstration of double-stranded RNA in cells infected with reovirus, poliomyelitis, and tick-borne encephalitis (TBE) viruses. Rabbit sera against poly(A)-poly(U) and poly(I)-poly(C) react specifically with double-stranded RNA. Double-stranded RNA is found in the cytoplasm of the cells infected with high multiplicities of poliomyelitis and tick-borne encephalitis viruses 3 hr postinoculation. In parallel, preparations were stained with sera against viral proteins. During one cycle of reproduction the dynamics of accumulation of double-stranded RNA and virus protein was synchronous both for poliomyelitis and tick-borne encephalitis viruses. When poliovirus-infected cells degenerated, the number of cells containing TBE virus double-stranded RNA decreased markedly while the proportion of cells containing virus protein remained high.
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ISSN:0042-6822
1096-0341
DOI:10.1016/0042-6822(74)90322-5