High-performance liquid chromatography analysis of purine nucleosides in human gingival crevicular fluid
Samples of human crevicular fluid were collected and analysed by high-performance liquid chromatography (HPLC) for purine nucleotides, nucleosides and bases. The complex chromatograms, had concentrations of compounds in two regions. Region (I) corresponded to purine bases and nucleosides, adenine an...
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Published in | Archives of oral biology Vol. 29; no. 8; pp. 607 - 610 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
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England
Elsevier Ltd
1984
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ISSN | 0003-9969 1879-1506 |
DOI | 10.1016/0003-9969(84)90129-8 |
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Abstract | Samples of human crevicular fluid were collected and analysed by high-performance liquid chromatography (HPLC) for purine nucleotides, nucleosides and bases. The complex chromatograms, had concentrations of compounds in two regions. Region (I) corresponded to purine bases and nucleosides, adenine and hypoxanthine were specifically identified. In region (II), where purine nucleotide mono- and diphosphates are generally observed, IMP and AMP were identified. In one subject with advanced periodontitis, additional compounds in region II were observed; crevicular fluid in gingivitis had the majority of their purine-containing compounds in region I. |
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AbstractList | Samples of human crevicular fluid were collected and analysed by high-performance liquid chromatography (HPLC) for purine nucleotides, nucleosides and bases. The complex chromatograms, had concentrations of compounds in two regions. Region (I) corresponded to purine bases and nucleosides, adenine and hypoxanthine were specifically identified. In region (II), where purine nucleotide mono- and diphosphates are generally observed, IMP and AMP were identified. In one subject with advanced periodontitis, additional compounds in region II were observed; crevicular fluid in gingivitis had the majority of their purine-containing compounds in region I. Samples of human crevicular fluid were collected and analysed by high-performance liquid chromatography (HPLC) for purine nucleotides, nucleosides and bases. The complex chromatograms, had concentrations of compounds in two regions. Region (I) corresponded to purine bases and nucleosides, adenine and hypoxanthine were specifically identified. In region (II), where purine nucleotide mono- and diphosphates are generally observed, IMP and AMP were identified. In one subject with advanced periodontitis, additional compounds in region II were observed; crevicular fluid in gingivitis had the majority of their purine-containing compounds in region I.Samples of human crevicular fluid were collected and analysed by high-performance liquid chromatography (HPLC) for purine nucleotides, nucleosides and bases. The complex chromatograms, had concentrations of compounds in two regions. Region (I) corresponded to purine bases and nucleosides, adenine and hypoxanthine were specifically identified. In region (II), where purine nucleotide mono- and diphosphates are generally observed, IMP and AMP were identified. In one subject with advanced periodontitis, additional compounds in region II were observed; crevicular fluid in gingivitis had the majority of their purine-containing compounds in region I. |
Author | Brecx, M. Rossomando, E.F. Jahngen, E.G.E. |
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Cites_doi | 10.3109/00016356309028193 10.1177/00220345810600030101 10.1111/j.1600-0765.1966.tb01874.x 10.1016/0003-2697(81)90325-0 10.3109/00016356309011240 10.1016/S0021-9673(00)85021-9 10.1016/S0022-4804(83)80012-2 10.1111/j.1600-0765.1966.tb01872.x 10.1080/01483918108064826 10.1016/S0021-9673(01)84111-X |
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SubjectTerms | Adenine - analysis Adenosine Monophosphate - analysis Chromatography, High Pressure Liquid Gingival Crevicular Fluid - metabolism Gingivitis - metabolism Humans Hypoxanthines - analysis Inosine Monophosphate - analysis Periodontitis - metabolism Purine Nucleosides - analysis Purine Nucleotides - analysis |
Title | High-performance liquid chromatography analysis of purine nucleosides in human gingival crevicular fluid |
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