High-performance liquid chromatography analysis of purine nucleosides in human gingival crevicular fluid

Samples of human crevicular fluid were collected and analysed by high-performance liquid chromatography (HPLC) for purine nucleotides, nucleosides and bases. The complex chromatograms, had concentrations of compounds in two regions. Region (I) corresponded to purine bases and nucleosides, adenine an...

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Published inArchives of oral biology Vol. 29; no. 8; pp. 607 - 610
Main Authors Jahngen, E.G.E., Brecx, M., Rossomando, E.F.
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 1984
Subjects
Adenine - analysis
Adenosine Monophosphate - analysis
Chromatography, High Pressure Liquid
Gingival Crevicular Fluid - metabolism
Gingivitis - metabolism
Humans
Hypoxanthines - analysis
Inosine Monophosphate - analysis
Periodontitis - metabolism
Purine Nucleosides - analysis
Purine Nucleotides - analysis
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ISSN0003-9969
1879-1506
DOI10.1016/0003-9969(84)90129-8

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Summary:Samples of human crevicular fluid were collected and analysed by high-performance liquid chromatography (HPLC) for purine nucleotides, nucleosides and bases. The complex chromatograms, had concentrations of compounds in two regions. Region (I) corresponded to purine bases and nucleosides, adenine and hypoxanthine were specifically identified. In region (II), where purine nucleotide mono- and diphosphates are generally observed, IMP and AMP were identified. In one subject with advanced periodontitis, additional compounds in region II were observed; crevicular fluid in gingivitis had the majority of their purine-containing compounds in region I.
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ISSN:0003-9969
1879-1506
DOI:10.1016/0003-9969(84)90129-8