Mass spectographic analysis of stimulated release of endogenous amino acids from rat hippocampal slices

Amino acids released from in vitro rat hippocampal slices following stimulation of the Schaffer's collateral pathway, were collected by micro-perfusion and analysed by chemical ionization mass spectrometry, with isotope ratiodetermination as the quantitative technique, through the use of stable...

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Bibliographic Details
Published inBrain research Vol. 212; no. 1; p. 194
Main Authors Spencer, H J, Tominez, G, Halpern, B
Format Journal Article
LanguageEnglish
Published Netherlands 11.05.1981
Subjects
Amino Acids - metabolism
Animals
Aspartic Acid - metabolism
Culture Techniques
Dominance, Cerebral - physiology
Glutamates - metabolism
Hippocampus - metabolism
Mass Spectrometry
Neural Pathways - metabolism
Rats
Synaptic Transmission
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Summary:Amino acids released from in vitro rat hippocampal slices following stimulation of the Schaffer's collateral pathway, were collected by micro-perfusion and analysed by chemical ionization mass spectrometry, with isotope ratiodetermination as the quantitative technique, through the use of stable, isotopically labeled internal standards. Stimulation at 10 pulses/sec resulted in a doubling of the release of aspartic acid over the resting level and about a 60% increase in glutamate release, which is in essential agreement with studies utilizing K+ depolarization-evoked release from slices.
ISSN:0006-8993
DOI:10.1016/0006-8993(81)90050-0