Medium-dependent structure modifications of reconstituting fibrin

Two main forms of fibrin fibrils (cross-striated and smooth) were obtained by reconstituting fibrin gels from fibrin monomer solution at various experimental conditions. The cross-banding tendency is strong at low temperature (0–7°) and also at 36–37°. At room temperature fibrin polymerization resul...

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Published inBiochimica et biophysica acta Vol. 236; no. 3; pp. 546 - 549
Main Authors Belitser, V.A., Manjakov, V.Ph, Varetskaja, T.V.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 29.06.1971
Subjects
Binding Sites
Biophysical Phenomena
Biophysics
Calcium Chloride
Fibrin
Fibrinogen
Gels
Hydrogen-Ion Concentration
Macromolecular Substances
Microscopy, Electron
Osmolar Concentration
Phosphates
Polymers
Temperature
Time Factors
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Summary:Two main forms of fibrin fibrils (cross-striated and smooth) were obtained by reconstituting fibrin gels from fibrin monomer solution at various experimental conditions. The cross-banding tendency is strong at low temperature (0–7°) and also at 36–37°. At room temperature fibrin polymerization resulted in smooth fibrils. The transition from one tendency to the other proved to be quite sharp. If formed at an ionic strength in the range of 0.15–0.21 (at 5°) the fibrils showed a clear periodicity, but at ionic strength 0.3 smooth fibrils appeared. By raising the pH from 6.8 to 7.4 at 37° a change of reconstituting fibrils from striated to smooth could be affected. A tentative explanation of the phenomenon observed is suggested.
Bibliography:ObjectType-Article-1
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ISSN:0005-2795
0006-3002
1879-2952
DOI:10.1016/0005-2795(71)90238-8