Quantitative slide micro-immunoenzymatic assay (micro-SIA) for antibodies to particulate and non-particulate antigens

A quantitative, minimized version of the slide immunoenzymatic assay (SIA) is described, which is performed with a 5 microliters drop of each reagent on a circular glass surface (3 mm in diameter) delimited by a thin layer of a hydrophobic material. Measurements are done directly, without disturbing...

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Bibliographic Details
Published inJournal of immunological methods Vol. 59; no. 1; p. 39
Main Authors Conway de Macario, E, Macario, A J, Jovell, R J
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.1983
Subjects
Animals
Antibodies - analysis
Antibodies, Bacterial - analysis
Antigen-Antibody Reactions
Humans
Immunoenzyme Techniques - instrumentation
Rabbits
Spectrophotometry - instrumentation
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Summary:A quantitative, minimized version of the slide immunoenzymatic assay (SIA) is described, which is performed with a 5 microliters drop of each reagent on a circular glass surface (3 mm in diameter) delimited by a thin layer of a hydrophobic material. Measurements are done directly, without disturbing the drop of substrate solution while the enzymatic reaction takes place, by means of a vertical beam spectrophotometer. The main features of this quantitative micro-SIA are its simplicity, reproducibility, virtual absence of background and applicability to a large variety of antigen-antibody systems.
ISSN:0022-1759
DOI:10.1016/0022-1759(83)90143-6