Fas gene promoter −670 polymorphism (A/G) is associated with cervical carcinogenesis

• Published in: Gynecologic oncology Vol. 98; no. 1; pp. 129 - 133
• Main Authors: Ueda, Masatsugu, Hung, Yao-Ching, Terai, Yoshito, Yamaguchi, Hiroyuki, Saito, Junko, Nunobiki, Osamu, Noda, Sadamu, Ueki, Minoru
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2005
• Subjects: Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - virology; Cell Line, Tumor; Cervical carcinogenesis; Cervical Intraepithelial Neoplasia - genetics; Cervical Intraepithelial Neoplasia - virology; Fas; fas Receptor - genetics; Female; Genetic Predisposition to Disease; Genotype; Humans; Papillomaviridae - classification; Polymerase Chain Reaction; Polymorphism; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; SIL; Uterine Cervical Neoplasms - genetics; Uterine Cervical Neoplasms - virology; Fas; Cervical carcinogenesis; SIL; Polymorphism
• ISSN: 0090-8258; 1095-6859
• DOI: 10.1016/j.ygyno.2005.04.001

To investigate the biological significance of single nucleotide polymorphism (SNP) at Fas gene promoter in cervical carcinogenesis. SNP at −670 of Fas gene promoter (A/G) together with human papillomavirus (HPV) types were examined in a total of 279 cervical smear samples and 8 human cervical squamous carcinoma cell lines using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) techniques. 49 patients with high-grade squamous intraepithelial lesion (HSIL) had higher frequency of high-risk HPV and GA + GG genotype than 167 with low-grade SIL (LSIL) and 63 controls. G allele frequency was also higher in HSIL than in LSIL and controls. There was an increased OR (6.00; CI, 1.32–27.37; P = 0.021) for GA + GG genotype in HSIL cases compared to controls among 96 patients with high-risk HPV. 7 of 8 cervical carcinoma cell lines also showed GA or GG genotype. Fas gene promoter −670 polymorphism (A/G) may be closely associated with cervical carcinogenesis in a Japanese population.