Bioassay of mosquito iridescent virus of Aedes taeniorhynchus in cell cultures of Aedes aegypti

A bioassay of mosquito iridescent virus (MIV) of Aedes taeniorhynchus was developed using cell cultures of Aedes aegypti. The dilution end point technique was based on the occurrence of cytopathic effects which were optimum at 31°C. Peleg's A. aegypti cell line was more sensitive and reliable t...

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Bibliographic Details
Published inJournal of invertebrate pathology Vol. 26; no. 2; pp. 205 - 212
Main Authors Webb, S.R., Paschke, J.D., Wagner, G.W., Campbell, W.R.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.09.1975
Subjects
Aedes - microbiology
Animals
Cell Line
Cytopathogenic Effect, Viral
DNA Viruses - growth & development
Insect Viruses - growth & development
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Summary:A bioassay of mosquito iridescent virus (MIV) of Aedes taeniorhynchus was developed using cell cultures of Aedes aegypti. The dilution end point technique was based on the occurrence of cytopathic effects which were optimum at 31°C. Peleg's A. aegypti cell line was more sensitive and reliable than Singh's A. aegypti cell line for infectivity titration of the “R” and “T” strains of MIV. The highest tissue culture infectivity dose 50s (TCID 50) were elicited by virion:cell ratios of approximately 10. TCID 50 titers were significantly reduced by virus neutralization with either homologous or heterologous antiserum to either RMIV or TMIV. The virus propagated in either cell line was not infectious to A. taeniorhynchus larvae, or to the respective cells from which the virus was produced. All plaque assay attempts were unsuccessful.
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ISSN:0022-2011
1096-0805
DOI:10.1016/0022-2011(75)90050-6