Antigen overlay technique for line immunoelectrophoresis and crossed-line immunoelectrophoresis of thermolabile antigens

A simple antigen overlay technique for line immunoelectrophoresis and crossed-line immunoelectrophoresis is described. A blind gel is overlaid with antigen-impregnated filter paper instead to mix antigens with molten agarose and to pour them in a uniform sample gel. This makes it easier in line and...

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Bibliographic Details
Published inJournal of immunological methods Vol. 51; no. 1; p. 29
Main Authors Guinet, R, Gabriel, S
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.1982
Subjects
Animals
Antigens, Fungal
Candida - immunology
Hot Temperature
Immune Sera - pharmacology
Immunoelectrophoresis - methods
Immunoelectrophoresis, Two-Dimensional - methods
Rabbits
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Summary:A simple antigen overlay technique for line immunoelectrophoresis and crossed-line immunoelectrophoresis is described. A blind gel is overlaid with antigen-impregnated filter paper instead to mix antigens with molten agarose and to pour them in a uniform sample gel. This makes it easier in line and crossed-line immunoelectrophoresis comparison of different antigen samples, simplifying technique as compared with the conventional method. Moreover, this technique is designated to obtain reproducible patterns from thermolabile proteins since they are unaffected by being heated.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(82)90378-7