Eosinophil leukocyte responses and hypersensitivity reactions in the Bordetella pertussis-treated mouse

• Published in: The Journal of allergy Vol. 46; no. 4; pp. 205 - 215
• Main Authors: Cohen, Sheldon G., Sapp, Theresa M., Chiampi, P.Nona
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.1970
• Subjects: Animals; Antibody Formation; Bordetella pertussis - immunology; Disease Models, Animal; Eosinophilia - etiology; Hypersensitivity - etiology; Injections, Intraperitoneal; Mice; Vaccines - pharmacology
• ISSN: 0021-8707
• DOI: 10.1016/0021-8707(70)90024-9

Studies were concerned with an evaluation of hypersensitivity as one factor in the emergence of eosinophilia from antigen-antibody complex formation. Investigative approaches combined two experimental procedures in susceptible CFW strain mice: (1) Bordetella pertussis treatment to effect models of histamine-serotonin sensitivity and anaphylactic responsiveness and (2) sensitization and intraperitoneal challenge with egg albumin (EA) to induce local eosinophil responses in cell populations sampled during the resultant inflammatory process. Groups of 10 animals each were studied for these developments in varied modifications of the basic procedure. These experimental factors included single priming and multiple immunization injections, 4, 10, and 20 day sensitization schedules, and peritoneal fluid recovery at 6 hour and at 4 day periods after intraperitoneal antigenic challenge. In eight paired series there were no significant differences in quantitated eosinophilia between the control and B. pertussis-treated animals. Eosinophil cell responses could not be related to: the nonspecific leukocytosis of inflammation or lymphocyte-related leukocytosis of B, pertussis; techniques favorable for effecting histamine responsive states, anaphylactic manifestations, or adjuvant immunizing effects of the vaccine; levels of 2-mercaptoethanol (2-ME)-sensitive hemagglutinating antibody; or the presence of precipitins to EA. A role for reaginic antibody reactivity in experimental eosinophilia is suggested but requires further study.