Rapid assays for adenylate cyclase and 3′, 5′ cyclic AMP phosphodiesterase activities. Simultaneous measurements of other pathways of ATP catabolism

This paper deals with a rapid assay of adenylate cyclase activity and 3′,5′ cyclic AMP phosphodiesterase activity which permits simultaneous measurements of other pathways of ATP catabolism. The separation of all the adenylic nucleotides was obtained by an electrophoresis on cellulose acetate 15 min...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 62; no. 2; pp. 417 - 425
Main Authors Delaage, Michel A., Bellon, Bernard N., Cailla, Hélène L.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.12.1974
Subjects
Adenosine Triphosphate - metabolism
Adenylyl Cyclases - analysis
Adenylyl Cyclases - metabolism
Animals
Cattle
Cellulose
Cyclic AMP
Electrophoresis
Fluorescence
Kinetics
Methods
Pancreas - enzymology
Phosphoric Diester Hydrolases - analysis
Rats
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Summary:This paper deals with a rapid assay of adenylate cyclase activity and 3′,5′ cyclic AMP phosphodiesterase activity which permits simultaneous measurements of other pathways of ATP catabolism. The separation of all the adenylic nucleotides was obtained by an electrophoresis on cellulose acetate 15 min at 50 V/cm with a fluorescent buffer pH 8.6. Before electrophoresis, the incubation sample was added with a carrier solution of nucleotides to allow their localization under uv light, by fluorescence inhibition. Each fraction was cut and dissolved in Bray's liquid for scintillation counting. This assay method is rapid, reliable, and sensitive, it is suitable either for research or for routine and clinical purposes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(74)90174-2