Isolation and purification of bovine and canine prothrombin

A procedure for isolation and purification of prothrombin, applicable to both the bovine and canine species is described. The preparations are of good yield and of very high specific activity, viz. 2400–3000 Iowa units per mg of protein and show a single symmetric component in both the analytical ul...

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Bibliographic Details
Published inBiochimica et biophysica acta Vol. 111; no. 1; pp. 174 - 180
Main Authors Moore, H.C., Lux, S.E., Malhotra, O.P., Bakerman, S., Carter, J.R.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 15.11.1965
Subjects
Animals
Blood Chemical Analysis
Blood Protein Electrophoresis
Cattle
Chemistry Techniques, Analytical
Dogs
In Vitro Techniques
Prothrombin
Ultracentrifugation
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Summary:A procedure for isolation and purification of prothrombin, applicable to both the bovine and canine species is described. The preparations are of good yield and of very high specific activity, viz. 2400–3000 Iowa units per mg of protein and show a single symmetric component in both the analytical ultracentrifuge and by moving-boundary electrophoresis. Since preparations of such high specific activity are not activated optimally BaCO 3-adsorbed serum, it is necessary to modify the two-stage prothrombin assay method to provide optimal activator activity.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/0304-4165(65)90484-8