Propagation of human fetal spongioblasts and astrocytes in dispersed cell cultures

Cultures consisting exclusively of monolayered immature astrocytes with superimposed migratory spongioblasts were prepared by trypsinization of human fetal brain of 4 1 2 − 5 months' gestation. Cell types were identified by classical neuroglial morphology and by staining reactions with classica...

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Bibliographic Details
Published inExperimental cell research Vol. 40; no. 3; pp. 554 - 569
Main Author Shein, H.M.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.12.1965
Subjects
Culture Techniques
Ectoderm - cytology
Ectoderm - metabolism
Fetus
Humans
Microscopy
Neuroglia
Staining and Labeling
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Summary:Cultures consisting exclusively of monolayered immature astrocytes with superimposed migratory spongioblasts were prepared by trypsinization of human fetal brain of 4 1 2 − 5 months' gestation. Cell types were identified by classical neuroglial morphology and by staining reactions with classical neuroglial metallic impregnation techniques. These characteristics remained stable in each cell type through serial passages during more than four months' cultivation. In addition to observations on the different growth patterns and characteristics of the two cell types, techniques were developed for preparation of pure cultures of astrocytes and for preparation of cell suspensions consisting predominantly of spongioblasts or of astrocytes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(65)90234-X