Effect of ovotransferrin and lactoferrins on Chlamydophila psittaci adhesion and invasion in HD11 chicken macrophages

• Published in: Veterinary research (Paris) Vol. 38; no. 5; pp. 729 - 739
• Main Authors: BEECKMAN, Delphine Sylvie Anne, VAN DROOGENBROECK, Caroline M. A. D, DE COCK, Bart J. A, VAN OOSTVELDT, Patrick, VANROMPAY, Daisy C. G
• Format: Journal Article
• Language: English
• Published: Les Ulis EDP Sciences 01.09.2007; BioMed Central
• Subjects: Animal biology; Animal genetics; Animals; Bacterial Adhesion - drug effects; Bacteriology; Biochemistry, Molecular Biology; Biological and medical sciences; Cell Behavior; Cell Line; Cellular Biology; Chickens; Chlamydia Infections - microbiology; Chlamydia Infections - prevention & control; Chlamydia Infections - veterinary; Chlamydophila psittaci - drug effects; Chlamydophila psittaci - growth & development; Chlamydophila psittaci - pathogenicity; Chlamydophila psittaci - physiology; Conalbumin - pharmacology; Dose-Response Relationship, Drug; Fundamental and applied biological sciences. Psychology; Genetics; Humans; Immunology; Lactoferrin - pharmacology; Life Sciences; Macrophages - microbiology; Microbiology; Microbiology and Parasitology; Miscellaneous; Molecular biology; Neurons and Cognition; Santé publique et épidémiologie; Farming animal; Chlamydiaceae; Poultry; Ovotransferrin; Chlamydia psittaci; Adhesion; Vertebrata; Lactoferrin; Chlamydia; Chlamydiales; Bacteria; Chicken; Aves; Veterinary; Chlamydophila psittaci; Macrophage; lactoferrin; ovotransferrin
• ISSN: 0928-4249; 1297-9716
• DOI: 10.1051/vetres:2007028

The effect of ovotransferrin (ovoTF), human lactoferrin (hLF) and bovine lactoferrin (bLF) on the obligate intracellular pathogen Chlamydophila (Cp.) psittaci was evaluated using a model of Buffalo Green Monkey kidney (BGM) cells and HD11 chicken macrophages as artificial hosts. Firstly, the effect of transferrins on the infectivity of the bacteria was evaluated. Pre-incubation of Cp. psittaci with 0.5 to 5 mg/mL ovoTF prior to infecting BGM cells significantly lowered the infection rate (P < 0.05). For both lactoferrins, the infection rate could only be reduced with 5 mg/mL, albeit not significantly as compared to the infection rate created by the untreated bacteria. Secondly, transferrins were tested for their ability to influence bacterial adhesion and entry in HD11 cells. Maximal non-cytotoxic and non-bactericidal concentrations of 0.05 mg/mL ovoTF and 0.5 mg/mL hLF and bLF were used. Overall, ovoTF was more effective than human and bovine LF in inhibiting bacterial irreversible attachment and cell entry and the latter was accompanied by a dose-dependent reduction of actin recruitment at the bacterial entry site. However, once bacteria had entered HD11 cells, transferrins had apparently no effect on intracellular replication. The present findings suggest a possible role for transferrins and especially ovoTF, in preventing avian Cp. psittaci infections.