Interference of AHL signal production in the phytophatogen Pantoea agglomerans as a sustainable biological strategy to reduce its virulence

• Published in: Microbiological research Vol. 285; p. 127781
• Main Authors: Cruz, Alba Amaro-da, Cabeo, Mónica, Durán-Viseras, Ana, Sampedro, Inmaculada, Llamas, Inmaculada
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.08.2024
• Subjects: Acyl-Butyrolactones - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Pantoea - genetics; Pantoea - metabolism; Pantoea - pathogenicity; Plant Diseases - microbiology; Plant Diseases - prevention & control; Plant growth-promoting bacteria; Plant pathogens; Quorum quenching; Quorum Sensing; Solanum lycopersicum - microbiology; Virulence; Plant growth-promoting bacteria; Quorum quenching; Quorum sensing; Plant pathogens
• ISSN: 0944-5013; 1618-0623; 1618-0623
• DOI: 10.1016/j.micres.2024.127781

Pantoea agglomerans is considered one of the most ubiquitous and versatile organisms that include strains that induce diseases in various crops and occasionally cause opportunistic infections in humans. To develop effective strategies to mitigate its impact on plant health and agricultural productivity, a comprehensive investigation is crucial for better understanding its pathogenicity. One proposed eco-friendly approach involves the enzymatic degradation of quorum sensing (QS) signal molecules like N-acylhomoserine lactones (AHLs), known as quorum quenching (QQ), offering potential treatment for such bacterial diseases. In this study the production of C4 and 3-oxo-C6HSL was identified in the plant pathogenic P. agglomerans CFBP 11141 and correlated to enzymatic activities such as amylase and acid phosphatase. Moreover, the heterologous expression of a QQ enzyme in the pathogen resulted in lack of AHLs production and the attenuation of the virulence by mean of drastically reduction of soft rot disease in carrots and cherry tomatoes. Additionally, the interference with the QS systems of P. agglomerans CFBP 11141 by two the plant growth-promoting and AHL-degrading bacteria (PGP-QQ) Pseudomonas segetis P6 and Bacillus toyonensis AA1EC1 was evaluated as a potential biocontrol approach for the first time. P. segetis P6 and B. toyonensis AA1EC1 demonstrated effectiveness in diminishing soft rot symptoms induced by P. agglomerans CFBP 11141 in both carrots and cherry tomatoes. Furthermore, the virulence of pathogen notably decreased when co-cultured with strain AA1EC1 on tomato plants.