Triglyceride biosynthesis from monoglycerides in isolated segments of intestinal mucosa Utilization of an ether analogue of 2-monostearin

• Published in: Biochimica et biophysica acta Vol. 98; no. 3; pp. 539 - 546
• Main Authors: Sherr, Stanley I., Treadwell, C.R.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.1965
• Subjects: Animals; Chromatography, Thin Layer; Glycerides - biosynthesis; Glycerides - metabolism; In Vitro Techniques; Intestinal Mucosa - metabolism; Intestine, Small - metabolism; Rats
• ISSN: 0005-2760; 0006-3002; 1879-145X
• DOI: 10.1016/0005-2760(65)90150-5

1. 1. Triglyceride synthesis from monoglycerides was investigated during uptake by everted segments of rat small intestine. 2. 2. 1-Monoglycerides are converted into triglycerides via a 1,3-diglyceride intermediate. 1,2-Diglycerides are also formed during this conversion in varying amounts depending on the experimental conditions. This indicates the existence of an equilibrium between the 1,2- and 1,3-diglyceride isomers. It could not be ascertained whether 1,3-diglycerides are directly acylated to form triglycerides or are first isomerized to a 1,2-diglyceride. 3. 3. 2-Monoglycerides are converted to triglycerides via a 1,2-diglyceride intermediate. Indirect proof of this was achieved by using the ether analogue of 2-monostearin which was also taken up by the mucosa and esterified.