Studies on glycoconjugate metabolism in developing skeletal muscle membranes

• Published in: Biochimica et biophysica acta Vol. 755; no. 1; pp. 56 - 64
• Main Authors: Clark, Gary F., Smith, P.Blaise
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 04.01.1983
• Subjects: Aging; Animals; Animals, Newborn; Cell Membrane - metabolism; Development; Female; Glycoconjugate; Glycolipids - metabolism; Glycoproteins - metabolism; Kinetics; Membrane Proteins - metabolism; Muscle Development; Muscles - metabolism; Rabbits; Sarcoplasmic Reticulum - enzymology; Sialyltransferases - metabolism; Sialytransferase; Skeletal muscle membrane; Transferases - metabolism; Skeletal muscle membrane; Sialytransferase; GT1; Development; GM1; Glycoconjugate; GM3; GD1A; GT1A; CMP-sialic acid; GT1B
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/0304-4165(83)90272-6

The postnatal development of mammalian skeletal muscle is characterized by changes in the properties of several key membrane glycoprotein enzymes and receptors. In the recent study, CMP-sialic acid: fetuin sialyltransferase and CMP-sialic acid: lactosylceramide sialytransferase activity was characterized in sarcolemma and sarcoplasmic reticulum membranes isolated from neonatal (0–1 week) and adult (8 week) rabbit skeletal muscle. CMP-sialic acid: fetuin sialytransferase decreased by a factor of 10 in sarcolemma and 6 in sarcoplasmic reticulum during development, whereas CMP-sialic acid: lactosylceramide sialytransferase activity decreased by a factor of 6 in sarcolemma and 18 in sarcoplasmic reticulum. The K m for CMP-sialic acid using the lipid acceptor declined during the development of sarcoplasmic reticulum (neonate vs. adult: 538 vs. 33 μM), but not in sarcolemma. The carbohydrate composition of sarcolemma was changed only with respect to total sialic acid content (neonate vs. adult: 67 vs. 44 nmol/mg). Similar analysis of sarcoplasmic reticulum carbohydrates showed decreases in total sialic acid, lipid-bound sialic acid, hexosamines and hexoses. The major ganglioside was GM3 for both types of membrane. No qualitative changes were observed in ganglioside composition comparing neonatal and adult membranes.