Spectral-domain phase microscopy

Broadband interferometry is an attractive technique for the detection of cellular motions because it provides depth-resolved phase information via coherence gating. We present a phase-sensitive technique called spectral-domain phase microscopy (SDPM). SDPM is a functional extension of spectral-domai...

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Bibliographic Details
Published inOptics letters Vol. 30; no. 10; p. 1162
Main Authors Choma, Michael A, Ellerbee, Audrey K, Yang, Changhuei, Creazzo, Tony L, Izatt, Joseph A
Format Journal Article
LanguageEnglish
Published United States 15.05.2005
Subjects
Animals
Cell Movement - physiology
Cells, Cultured
Chick Embryo
Equipment Design
Equipment Failure Analysis
Microscopy, Interference - instrumentation
Microscopy, Interference - methods
Microscopy, Phase-Contrast - instrumentation
Microscopy, Phase-Contrast - methods
Myocardial Contraction - physiology
Myocytes, Cardiac - cytology
Myocytes, Cardiac - physiology
Tomography, Optical Coherence - instrumentation
Tomography, Optical Coherence - methods
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Summary:Broadband interferometry is an attractive technique for the detection of cellular motions because it provides depth-resolved phase information via coherence gating. We present a phase-sensitive technique called spectral-domain phase microscopy (SDPM). SDPM is a functional extension of spectral-domain optical coherence tomography that allows for the detection of nanometer-scale motions in living cells. The sensitivity of the technique is demonstrated, and its calibration is verified. A shot-noise limit to the displacement sensitivity of this technique is derived. Measurement of cellular dynamics was performed on spontaneously beating cardiomyocytes isolated from chick embryos.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.30.001162