Molecular complexity of Paramecium symbiont lambda deoxyribonucleic acid: Evidence for the presence of a multicopy genome

DNA was isolated from highly purified symbiont lambda particles of Paramecium aurelia. Renaturation kinetic data revealed a molecular size of 0.71 × 10 9 daltons. Analytical complexity estimated from chemical data was 7.5 × 10 9 daltons. These values correspond to about ten copies of the genome per...

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Bibliographic Details
Published inJournal of molecular biology Vol. 73; no. 1; pp. 93,IN25,97 - 96,IN25,108
Main Authors Soldo, A.T., Godoy, G.A.
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 1973
Subjects
Adenine - analysis
Animals
Centrifugation, Density Gradient
Cytosine - analysis
DNA - analysis
Formaldehyde
Guanine - analysis
Hot Temperature
Inclusion Bodies - analysis
Kinetics
Microscopy, Electron
Microscopy, Phase-Contrast
Molecular Weight
Nucleic Acid Denaturation
Nucleic Acid Renaturation
Paramecium - analysis
Rickettsiaceae - analysis
Spectrophotometry
Thymine - analysis
T m
melting point (defined as that temperature at which half the total increase in absorbancy at 260 nm has occurred)
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Summary:DNA was isolated from highly purified symbiont lambda particles of Paramecium aurelia. Renaturation kinetic data revealed a molecular size of 0.71 × 10 9 daltons. Analytical complexity estimated from chemical data was 7.5 × 10 9 daltons. These values correspond to about ten copies of the genome per lambda particle. The relationship of symbiont lambda particles to bacteria and cellular organelles, i.e. chloroplasts, mitochondria and kinetosomes, is discussed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-2836
1089-8638
DOI:10.1016/0022-2836(73)90161-7