Copper Staining/Labeling and Scanning Electrochemical Microscopy Readout of Proteins on Poly(vinylidene difluoride) Membranes

Scanning Electrochemical Microscopy (SECM) is used in combination with copper labeling to visualize roteins on surfaces. Proteins are adsorbed on a poly(vinylidene difluoride) (PVDF) membrane and stained using a standard protocol involving copper salts. The latter are then reduced to copper metal an...

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Bibliographic Details
Published inChimia Vol. 59; no. 3
Main Authors Maurizio Carano, Niels Lion, Hubert H. Girault
Format Journal Article
LanguageEnglish
Published Swiss Chemical Society 01.03.2005
Subjects
Copper staining
Membrane
Poly vinylidene difluoride
Protein detection
Scanning electrochemical microscopy secm
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Summary:Scanning Electrochemical Microscopy (SECM) is used in combination with copper labeling to visualize roteins on surfaces. Proteins are adsorbed on a poly(vinylidene difluoride) (PVDF) membrane and stained using a standard protocol involving copper salts. The latter are then reduced to copper metal and further detected by SECM with ferrocene methanol as a redox mediator in aqueous solution. During the SECM scan, the potential is held at a value at which the oxidation of the redox mediator occurs and a positive feedback current is detected when scanning over copper clusters. A negative feedback is observed elsewhere.
ISSN:0009-4293
2673-2424
DOI:10.2533/000942905777676849