Zinc finger domain of Su(Hw) protein is required for the formation of functional Su(Hw)-dependent insulator complex

This study is devoted to clarifying the role of Mod(mdg4)-67.2 and Su(Hw) proteins in the interaction between Su(Hw)-dependent insulator complexes and identifying the specific domains of the Su(Hw) protein required for insulation or mutual neutralization of insulators. Using genetic techniques and e...

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Published inDoklady. Biochemistry and biophysics Vol. 469; no. 1; pp. 247 - 252
Main Authors Golovnin, A. K., Molodina, V. V., Georgiev, P. G., Melnikova, L. S.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.07.2016
Springer Nature B.V
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Abstract This study is devoted to clarifying the role of Mod(mdg4)-67.2 and Su(Hw) proteins in the interaction between Su(Hw)-dependent insulator complexes and identifying the specific domains of the Su(Hw) protein required for insulation or mutual neutralization of insulators. Using genetic techniques and experiments in yeast two-hybrid system, we have demonstrated that the zinc finger domain of the Su(Hw) protein is involved in forming a functional insulator complex and cannot be replaced with the DNA-binding domain of the GAL4 protein.
AbstractList This study is devoted to clarifying the role of Mod(mdg4)-67.2 and Su(Hw) proteins in the interaction between Su(Hw)-dependent insulator complexes and identifying the specific domains of the Su(Hw) protein required for insulation or mutual neutralization of insulators. Using genetic techniques and experiments in yeast two-hybrid system, we have demonstrated that the zinc finger domain of the Su(Hw) protein is involved in forming a functional insulator complex and cannot be replaced with the DNA-binding domain of the GAL4 protein.
Author Molodina, V. V.
Golovnin, A. K.
Georgiev, P. G.
Melnikova, L. S.
Author_xml – sequence: 1
  givenname: A. K.
  surname: Golovnin
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  surname: Melnikova
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  organization: Institute of Gene Biology, Russian Academy of Sciences
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Snippet This study is devoted to clarifying the role of Mod(mdg4)-67.2 and Su(Hw) proteins in the interaction between Su(Hw)-dependent insulator complexes and...
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SubjectTerms Animals
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Deoxyribonucleic acid
DNA
Drosophila
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Genetic research
Life Sciences
Microtubule-Associated Proteins - genetics
Microtubule-Associated Proteins - metabolism
Molecular Biology
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Proteins
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Repressor Proteins - genetics
Repressor Proteins - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
Two-Hybrid System Techniques
Yeast
Zinc Fingers
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Title Zinc finger domain of Su(Hw) protein is required for the formation of functional Su(Hw)-dependent insulator complex
URI https://link.springer.com/article/10.1134/S1607672916040049
https://www.ncbi.nlm.nih.gov/pubmed/27599504
https://www.proquest.com/docview/1817059278
https://search.proquest.com/docview/1817847072
https://search.proquest.com/docview/1827933850
Volume 469
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