Zinc finger domain of Su(Hw) protein is required for the formation of functional Su(Hw)-dependent insulator complex

• Published in: Doklady. Biochemistry and biophysics Vol. 469; no. 1; pp. 247 - 252
• Main Authors: Golovnin, A. K., Molodina, V. V., Georgiev, P. G., Melnikova, L. S.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.07.2016; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Deoxyribonucleic acid; DNA; Drosophila; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Genetic research; Life Sciences; Microtubule-Associated Proteins - genetics; Microtubule-Associated Proteins - metabolism; Molecular Biology; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Proteins; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Repressor Proteins - genetics; Repressor Proteins - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism; Two-Hybrid System Techniques; Yeast; Zinc Fingers
• ISSN: 1607-6729; 1608-3091
• DOI: 10.1134/S1607672916040049

This study is devoted to clarifying the role of Mod(mdg4)-67.2 and Su(Hw) proteins in the interaction between Su(Hw)-dependent insulator complexes and identifying the specific domains of the Su(Hw) protein required for insulation or mutual neutralization of insulators. Using genetic techniques and experiments in yeast two-hybrid system, we have demonstrated that the zinc finger domain of the Su(Hw) protein is involved in forming a functional insulator complex and cannot be replaced with the DNA-binding domain of the GAL4 protein.