Mechanisms of polyclonal B-cell activation in autoimmune B6- lpr/lpr mice
The influence of the lpr gene on spontaneous and lipopolysaccharide (LPS)-induced immunoglobulin production was studied in B6 mice homozygous for the mutant lpr gene (B6- lpr/lpr). Male and female mice of this congenic strain were followed for 1 year and sera serially tested by the enzyme-linked imm...
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Published in | Cellular immunology Vol. 84; no. 1; pp. 22 - 31 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier Inc
01.03.1984
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Subjects | |
Online Access | Get full text |
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Summary: | The influence of the
lpr gene on spontaneous and lipopolysaccharide (LPS)-induced immunoglobulin production was studied in B6 mice homozygous for the mutant
lpr gene (B6-
lpr/lpr). Male and female mice of this congenic strain were followed for 1 year and sera serially tested by the enzyme-linked immunosorbent assay (ELISA) for the production of antibodies to single-stranded DNA (anti-sDNA), immunoglobulin (anti-lgG), and keyhole limpet hemocyanin (anti-KLH), models of autoantibody and non-autoantibody responses, respectively. Female B6-
lpr/lpr mice demonstrated marked spontaneous responses to all three antigens; the responses of male B6-
lpr/lpr mice were significantly lower but still exceeded those of the congenic B6-+/+ controls. These results demonstrate a generalized influence of sex on
lpr associated responses. To determine whether this sex difference could be demonstrated with other forms of B-cell activation, young B6-+/+ and B6-
lpr/lpr male and female mice were immunized with lipopolysaccharide and the induced responses determined. This immunization caused significant increases in the IgM response only. The levels of the induced responses produced after LPS treatment were comparable for +/+ and
lpr/lpr mice. These results indicate that the enhanced responsiveness of female mice to
lpr action is not reflected in the polyclonal response to LPS, which, furthermore, was unaffected by the presence of
lpr. The differential influence of sex on
lpr and LPS-induced responses and their apparent independence suggests that
lpr and LPS promote B-cell activation by dissimilar mechanisms. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0008-8749 1090-2163 |
DOI: | 10.1016/0008-8749(84)90073-X |