Distribution of arrestin-like protein and β-subunit of GTP-binding proteins in quail choroid plexuses
Monoclonal antibodies (Mabs) directed against retinal arrestin (S-antigen) were used to detect and characterize this protein in choroid plexus (CP) of quails maintained during eight days, either under long-day photoperiods or in constant darkness. Immunocytochemistry and Western blotting confirmed t...
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Published in | Cellular signalling Vol. 3; no. 5; pp. 461 - 472 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Inc
1991
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Subjects | |
Online Access | Get full text |
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Summary: | Monoclonal antibodies (Mabs) directed against retinal arrestin (S-antigen) were used to detect and characterize this protein in choroid plexus (CP) of quails maintained during eight days, either under long-day photoperiods or in constant darkness. Immunocytochemistry and Western blotting confirmed the presence and the distribution of an arrestin-like protein in quail CP. Arrestin-like immunoreactivities in CP were compared with those obtained with Mabs to
β
36-subunit of G proteins (Gβ), α-subunit of transducin and rhodopsin. Rhodopsin-like and transductin-like proteins could not be detected in choroidal cells, whereas intense positive reactions were observed with anti-Gβ and anti-arrestin Mabs. The strongest immunoreactivities were found in choroidal ependymocytes of the lateral and IIIrd ventricles. In CP epithelial cells lining the IVth ventricle, very weak or no immunoreactivity could be detected with Mabs to arrestin, while Mab against Gβ subunit always provided a positive reaction. In quails maintained in constant darkness, arrestin- and
Gβ-immunoreactivities of CP epithelial cells displayed changes in cellular distribution and intensity (decrease or disappearance of the immunoreactions). The strong arrestin-like immunoreaction located in the apical region of ependymocytes suggests the preferential association of the protein with choroidal microvilli and a possible role in cerebrospinal fluid production assumed by CP cells. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0898-6568 1873-3913 |
DOI: | 10.1016/0898-6568(91)90076-7 |