Localization of mouse satellite DNA in constitutive heterochromatin

Sonication of nuclei from the liver and brain of male mice yields three chromatin fractions with different sedimentation characteristics: a constitutive heterochromatin fraction which sediments at 3500 g, an intermediate chromatin fraction which sediments at 78,000 g and a euchromatin fraction which...

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Bibliographic Details
Published inExperimental cell research Vol. 59; no. 1; pp. 69 - 75
Main Authors Yasmineh, W.G., Yunis, J.J.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 1970
Subjects
Animals
Brain Chemistry
Cell Nucleus - analysis
Centrifugation, Density Gradient
Chromosomes - analysis
DNA - analysis
Electrophoresis
Heterochromatin - analysis
Liver - analysis
Male
Mice
Staining and Labeling
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Summary:Sonication of nuclei from the liver and brain of male mice yields three chromatin fractions with different sedimentation characteristics: a constitutive heterochromatin fraction which sediments at 3500 g, an intermediate chromatin fraction which sediments at 78,000 g and a euchromatin fraction which is recovered by precipitation with ethanol. Cesium chloride gradient centrifugation of DNA extracted from the three fractions revealed that the DNA from the constitutive heterochromatin is composed primarily of satellite DNA and represents in excess of 70% of the satellite DNA of the genome. The implications of this findings are discussed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(70)90624-5