Phylogeny of Eleusine (Poaceae: Chloridoideae) based on nuclear ITS and plastid trnT– trnF sequences

• Published in: Molecular phylogenetics and evolution Vol. 35; no. 2; pp. 395 - 419
• Main Authors: Neves, Susana S., Swire-Clark, Ginger, Hilu, Khidir W., Baird, Wm. Vance
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2005
• Subjects: Base Sequence; Chloridoideae; Consensus Sequence; DNA, Plant - genetics; DNA, Ribosomal - genetics; Eleusine; Eleusine - classification; Eleusine - genetics; Finger millet; Nuclear ITS; Paralogues; Phylogeny; Plastid trnT– trnF; Poaceae; Poaceae - classification; Poaceae - genetics; Polymorphism, Genetic; Polyploidy; Pseudogenes; RNA, Plant - genetics; RNA, Ribosomal, 16S - genetics; Plastid trnT– trnF; Paralogues; Pseudogenes; Eleusine; Nuclear ITS; Poaceae; Chloridoideae; Finger millet; Phylogeny
• ISSN: 1055-7903; 1095-9513
• DOI: 10.1016/j.ympev.2004.12.005

Phylogenetic relationships in the genus Eleusine (Poaceae: Chloridoideae) were investigated using nuclear ITS and plastid trnT– trnF sequences. Separate and combined data sets were analyzed using parsimony, distance, and likelihood based methods, including Bayesian. Data congruence was examined using character and topological measures. Significant data heterogeneity was detected, but there was little conflict in the topological substructure measures for triplets and quartets, and resolution and clade support increased in the combined analysis. Data incongruence may be a result of noise and insufficient information in the slower evolving trnT– trnF. Monophyly of Eleusine is strongly supported in all analyses, but basal relationships in the genus remain uncertain. There is good support for a CAIK clade ( E. coracana subsp. coracana and africana, E. indica, and E. kigeziensis), with E. tristachya as its sister group. Two putative ITS homeologues (A and B loci) were identified in the allotetraploid E. coracana; the ‘B’ locus sequence type was not found in the remaining species. Eleusine coracana and its putative ‘A’ genome donor, the diploid E. indica, are confirmed close allies, but sequence data contradicts the hypothesis that E. floccifolia is its second genome donor. The ‘B’ genome donor remains unidentified and may be extinct.