Truncated erythropoietin receptor in a murine erythroleukemia cell line

• Published in: The international journal of biochemistry & cell biology Vol. 28; no. 2; pp. 175 - 181
• Main Authors: Bittorf, Thomas, Busfield, Samantha J., Peter Klinken, S., Tilbrook, Peta A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.02.1996
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Cell Line, Transformed; Cell Transformation, Viral - physiology; Erythropoietin receptor; Friend murine leukemia virus - physiology; Leukemia, Erythroblastic, Acute - metabolism; Leukemia, Erythroblastic, Acute - virology; Mice; Molecular Sequence Data; Murine erythroleukemia; Protein Structure, Tertiary; Receptors, Erythropoietin - chemistry; RT; epo; MIEL; epo-R; Erythropoietin receptor; ER; PCR; Murine erythroleukemia
• ISSN: 1357-2725; 1878-5875
• DOI: 10.1016/1357-2725(95)00128-X

The Friend spleen focus forming virus produces a 55 kDa envelope glycoprotein which associates with the erythropoietin receptor. We compared the erythropoietin receptor in Friend virus transformed marine erythroleukemic NN and 707 cell lines with the J2E erythroid line generated by the J2 retrovirus. Reverse transcriptase PCR was used to determine transcript size. Erythropoietin receptor cDNAs were then sequenced and protein products analysed by Western blotting and immunoprecipitation. We show here that the F4N marine erythroleukemic cell line had an enlarged erythropoietin receptor mRNA. In contrast, the 707 and J2E cell lines had normal sized transcripts for the receptor. Sequence analysis of the receptor in F4N cells revealed that introns which separate the exons coding for the cytoplasmic domain of the receptor were retained in these transcripts. As a consequence, a premature stop codon had been introduced, leaving only four amino acids in the intracellular portion of the receptor molecule. The normal erythropoietin receptor is approx. 66–70 kDa, but immunoprecipitation of [ 35S]methionine/cysteine labelled cell lysates with an antibody to the amino-termiuus of the erythropoietin receptor identified a truncated 37 kDa protein in F4N cells. Despite the severe carboxy-terminal truncation of the erythropoietin receptor, F4N cells continued to proliferate like the other murine erythroleukemia cell lines. This study shows that failure to remove introns from the erythropoietin receptor mRNA in F4N cells has resulted in the production of a smaller protein with virtually no cytoplasmic domain.