Maintaining and Monitoring the Defined Microbiota Status of Gnotobiotic Rodents

Gnotobiotic (germfree, defined colonized) rodents have become powerful tools to advance our understanding of the host–microbiome relationship. However, the maintenance and ultimately the monitoring of gnotobiotic rodents is a critical, labor-intensive, and costly process (e.g., sterility, not absenc...

Full description

Saved in:
Bibliographic Details
Published inILAR journal Vol. 56; no. 2; pp. 241 - 249
Main Authors Nicklas, Werner, Keubler, Lydia, Bleich, André
Format Journal Article
LanguageEnglish
Published England Oxford University Press 31.08.2015
Subjects
Animals
Germ-Free Life
Microbiota - physiology
Models, Animal
Rats
Rodentia - microbiology
gnotobiotic
monitoring
germfree
Online AccessGet full text

Cover

More Information
Summary:Gnotobiotic (germfree, defined colonized) rodents have become powerful tools to advance our understanding of the host–microbiome relationship. However, the maintenance and ultimately the monitoring of gnotobiotic rodents is a critical, labor-intensive, and costly process (e.g., sterility, not absence of specific pathogens, must be demonstrated in germfree animals). Here, we provide information on the housing and maintenance of gnotobiotic animals, elucidate prophylactic measurements to avoid contamination, and make specific recommendations for sampling procedures, sampling frequencies, and test methods.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-1
ISSN:1084-2020
1930-6180
DOI:10.1093/ilar/ilv029