A hemoprotein from azotobacter containing non-heme iron: Isolation and crystallization

A new type of hemoprotein was isolated from extracts of Azotobacter vinelandii using a heat step, application of the protamine-cellulose phosphate procedure, and precipitation with MgCl 2. Crystallization was achieved using low concentration of MgCl 2. The protein was shown to be a unique cytochrome...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 54; no. 4; pp. 1274 - 1281
Main Authors Bulen, W.A., LeComte, J.R., Lough, S.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.10.1973
Subjects
Azotobacter - analysis
Crystallization
Cytochromes - analysis
Electrophoresis, Polyacrylamide Gel
Heme - analysis
Hemeproteins - analysis
Hemeproteins - isolation & purification
Iron - analysis
Oxidation-Reduction
Spectrophotometry
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Summary:A new type of hemoprotein was isolated from extracts of Azotobacter vinelandii using a heat step, application of the protamine-cellulose phosphate procedure, and precipitation with MgCl 2. Crystallization was achieved using low concentration of MgCl 2. The protein was shown to be a unique cytochrome of the b type containing up to 20% non-heme iron but no “acid labile sulfur”. Both the heme and non-heme iron appear to be functional redox components.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(73)91125-X