Synthesis of [ 14C]coproporphyrin III by yeast cell-free extracts

The procedure described utilizes a crude cell-free extract from the yeast Saccharomyces cerevisiae as enzymatic source for the synthesis of coproporphyrin III from [ 14C]δ-aminolevulinic acid with a high yield of conversion (⋍60%). Both specific radioactivity and total radioactivity of coproporphyri...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 126; no. 1; pp. 81 - 85
Main Authors Chambon, Henriette, Labbe, Pierre
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.10.1982
Subjects
Aminolevulinic Acid - metabolism
Cell-Free System - metabolism
Chromatography
Coproporphyrins - biosynthesis
Coproporphyrins - isolation & purification
Methods
Porphyrins - biosynthesis
Protoporphyrins - biosynthesis
Saccharomyces cerevisiae - metabolism
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Summary:The procedure described utilizes a crude cell-free extract from the yeast Saccharomyces cerevisiae as enzymatic source for the synthesis of coproporphyrin III from [ 14C]δ-aminolevulinic acid with a high yield of conversion (⋍60%). Both specific radioactivity and total radioactivity of coproporphyrin III can be adjusted fairly well. This procedure is not time consuming for yeast acellular extracts or porphyrin ester preparations. The acellular extracts can be stored frozen (−30°C) for at least 1 year without loss of enzymatic activity. The same procedure can be used for [ 14C]protoporphyrin preparation.
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SourceType-Scholarly Journals-1
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ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(82)90111-7