Endophytic fungi of teak leaves Tectona grandis L. and rain tree leaves Samanea saman Merr

• Published in: World journal of microbiology & biotechnology Vol. 22; no. 5; pp. 481 - 486
• Main Authors: Chareprasert, Sukanyanee, Piapukiew, Jittra, Thienhirun, Surang, Whalley, Anthony J. S, Sihanonth, Prakitsin
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.05.2006; Springer; Springer Nature B.V
• Subjects: Alternaria; Antimicrobial; Bacillus subtilis; Bacteria; Biological and medical sciences; Biotechnology; Candida; Colletotrichum; E coli; Endophytes; Escherichia coli; Fundamental and applied biological sciences. Psychology; Fungi; Fusarium; Leaves; Mycelia sterilia; Penicillium; Phomopsis; Plant species; Rain; Rainy season; Samanea; Schizophyllum commune; Staphylococcus aureus; Tectona; Tectona grandis; Trees; Xylariaceae; Antimicrobial; Plant leaf; Antimicrobial agent; Tectona; endophytes; Fungi; Samanea; Verbenaceae; Dicotyledones; Angiospermae; Endophyte; Spermatophyta; Tectona grandis; Thallophyta
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-005-9060-x

In order to study the foliar endophytes from teak (Tectona grandis L.) and rain tree (Samanea saman Merr.) growing in the campus of Chulalongkorn University, healthy leaves were collected at two-monthly intervals during January to December. The number of genera and species, together with their colonization frequency (CF%) in mature teak and rain tree leaves were greater than those in the young leaves. More endophytic isolates in the leaves of both trees were recovered during the rainy season. The fungal genera found in both young and mature teak leaves were Alternaria, Colletotrichum, Nigrospora, Phomopsis and mycelia sterilia. Phomopsis was the dominant genus in both young (newly emerged) and mature leaves. Fusarium, Penicillium, Schizophyllum commune and members of the Xylariaceae were found only in mature leaves. For the rain tree leaves, species of Phomopsis and mycelia sterilia were found in both young newly emerged and mature leaves. Colletotrichum and Penicillium were found only in mature leaves, whereas Nigrospora was found only in young newly emerged leaves. In this study, Phomopsis was the dominant genus in the leaves of both tree species. A total of 37 isolates of endophytic fungi isolated from teak and rain tree leaves were tested for the production of antimicrobial activities. Out of these, 18 isolates could produce inhibitory substances effective against Bacillus subtilis, Staphylococcus aureus and Escherichia coli and 3 isolates inhibited growth of Candida albicans in vitro.