The biochemical activities of rabbit alveolar macrophages during phagocytosis

The biochemical activities of non-induced rabbit alveolar macrophages during phagocytosis were studied. Serum was required for maximal phagocytosis of Staphylococcus albus. A 40 per cent increase in respiratory activity of macrophages during phagocytosis was noted. 14C-1-glucose, 14C-6-glucose, 14C-...

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Published inExperimental cell research Vol. 40; no. 3; pp. 456 - 468
Main Authors Ouchi, E., Selvaraj, R.J., Sbarra, A.J.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.12.1965
Subjects
Animals
Blood
Carbon Isotopes - metabolism
Citric Acid Cycle
Cyanides - pharmacology
Dactinomycin - pharmacology
Dinitrophenols - pharmacology
Fluorides - pharmacology
Glucose - metabolism
Hydrogen-Ion Concentration
In Vitro Techniques
Iodoacetates - pharmacology
Lactates - metabolism
Lung
Macrophages - metabolism
Methylene Blue - pharmacology
Oxygen Consumption
Phagocytosis
Phenazines - pharmacology
Rabbits
Temperature
Vitamin K - pharmacology
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Summary:The biochemical activities of non-induced rabbit alveolar macrophages during phagocytosis were studied. Serum was required for maximal phagocytosis of Staphylococcus albus. A 40 per cent increase in respiratory activity of macrophages during phagocytosis was noted. 14C-1-glucose, 14C-6-glucose, 14C-1-pyruvate and 14C-3-pyruvate were oxidized significantly more by phagocytizing cells. Anaerobiosis inhibits phagocytosis. NaF and iodoacetate did not inhibit phagocytosis but did inhibit glycolysis. Malonate, fluoroacetate, cyanide, antimycin A and amytal failed to affect particle uptake although a significant decrease in respiration was observed. Uncoupling agent, 2,4 dinitrophenol stimulated oxygen uptake, but did not affect particle uptake. The effects of adding electron acceptors were examined. No effects were noted under anaerobic conditions. Phenazine methosulfate, menadione bisulfite and methylene blue increased 14C-1-glucose and 14C-6-glucose oxidations and respiration. At concentrations above 0.075 m M for phenazine methosulfate and 0.05 m M for menadione bisulfite the increased respiratory activity associated with phagocytosis was diminished and phagocytosis was also inhibited.
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ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(65)90226-0