Evolution underground: A molecular phylogenetic investigation of Australian burrowing freshwater crayfish (Decapoda: Parastacidae) with particular focus on Engaeus Erichson

• Published in: Molecular phylogenetics and evolution Vol. 50; no. 3; pp. 580 - 598
• Main Authors: Schultz, Mark B., Smith, Sarah A., Horwitz, Pierre, Richardson, Alastair M.M., Crandall, Keith A., Austin, Christopher M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2009
• Subjects: 16S; Animals; Astacoidea - classification; Astacoidea - genetics; Australia; Bayes Theorem; Biogeography; Burrowing freshwater crayfish; Cell Nucleus - genetics; DNA, Mitochondrial - genetics; Evolution; Evolution, Molecular; GAPDH; Genetic Speciation; Mitochondrial ribosomal; Nuclear protein-coding DNA; Phylogenetics; Phylogeny; Relaxed molecular clock; RNA, Ribosomal, 16S - genetics; Sequence Alignment; Sequence Analysis, DNA; Systematics; Australia; Burrowing freshwater crayfish; Relaxed molecular clock; Nuclear protein-coding DNA; Systematics; Mitochondrial ribosomal; Phylogenetics; Biogeography; Evolution; Australia; GAPDH; 16S
• ISSN: 1055-7903; 1095-9513
• DOI: 10.1016/j.ympev.2008.11.025

Phylogenetic relationships and species boundaries of Australian burrowing freshwater crayfish belonging to the genera Engaeus, Engaewa, Geocharax, Gramastacus and Tenuibranchiurus are investigated using combined mitochondrial and nuclear DNA sequence data and Bayesian and Maximum Parsimony methods. Phylogenies are statistically compared to previously published hypotheses. Engaeus, Engaewa, Geocharax, Gramastacus and Tenuibranchiurus form a strongly supported monophyletic clade. This grouping is independently supported by morphology but unites geographically highly disjunct lineages. Our data show two cryptic species in Geocharax, one cryptic species in Gramastacus and two cryptic species within the highly divergent Engaeus lyelli lineage. Using a Bayesian relaxed molecular clock method, the 16S rDNA data show generic-level diversification coinciding with the transition from a wet to arid palaeoclimate near the mid Miocene.