Monitoring of blood levels of cyclosporine in renal and cardiac transplant recipients — Comparison of HPLC to Incstar CYCLO-Trac SP RIA
Cyclosporine in whole blood samples from renal and cardiac transplant recipients was measured by high-performance liquid chromatography (HPLC) and by CYCLO-Trac SP specific radioimmunoassay (RIA). The fresh samples assayed by RIA were remeasured in a second laboratory after storage at −20°C for two...
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Published in | Clinical biochemistry Vol. 24; no. 1; pp. 49 - 53 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.02.1991
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Subjects | |
Online Access | Get full text |
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Summary: | Cyclosporine in whole blood samples from renal and cardiac transplant recipients was measured by high-performance liquid chromatography (HPLC) and by CYCLO-Trac SP specific radioimmunoassay (RIA). The fresh samples assayed by RIA were remeasured in a second laboratory after storage at −20°C for two to six months and good interlaboratory agreement was obtained on the 59 samples assayed (
y = 0.926× + 14.8 μg/L,
r = 0.982). The calibration curve for RIA was not influenced by fresh whole blood, hemolyzed whole blood or serum matrix from normal volunteers. However, comparison of the RIA results from patient samples with those from an HPLC procedure showed that RIA values averaged about double those measured by HPLC. The difference is attributable to differences between the blood matrix of transplant and nontransplant subjects, rather than exclusively to the apparent nonspecific nature of the antibody. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0009-9120 1873-2933 |
DOI: | 10.1016/0009-9120(91)90189-L |