Fast atom bombardment-mass spectrometric identification of molecular species of platelet-activating factor produced by stimulated human polymorphonuclear leukocytes

Fast atom bombardment mass spectrometry was used to identify molecular species of platelet-activating factor (PAF) produced by stimulated human neutrophilic polymorphonuclear leukocytes. Normal and reverse-phase high performance liquid chromatography were employed to separate the individual regions...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 129; no. 3; pp. 868 - 876
Main Authors Weintraub, Susan T., Ludwig, Janet C., Mott, Glen E., McManus, Linda M., Lear, Cynthia, Pinckard, R.Neal
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 28.06.1985
Subjects
Chromatography, High Pressure Liquid
Humans
Mass Spectrometry
Neutrophils - analysis
Neutrophils - drug effects
Platelet Activating Factor - analysis
Spectrophotometry, Atomic
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Summary:Fast atom bombardment mass spectrometry was used to identify molecular species of platelet-activating factor (PAF) produced by stimulated human neutrophilic polymorphonuclear leukocytes. Normal and reverse-phase high performance liquid chromatography were employed to separate the individual regions with PAF activity prior to mass spectrometric analysis. The following alkyl chain homologs of acetyl glyceryl ether phosphorylcholine (AGEPC) were found: C16:0, C17:0, C18:0 and C18:1. There was also evidence for the presence of the C15:0 homolog, as well as other species which have not yet been identified.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(85)91972-2