Observations on the dissociation of oxy-, deoxy- and ferrihaemoglobin in relation to pH and ionic strength by the Sephadex method
There are significant differences in the behaviour of oxy-, deoxy- and ferrihaemoglobin when studied under dissociating conditions by the Sephadex method. The dissociation of oxyhaemoglobin appears to be insensitive to a change of pH from 7.0 to 5.5, in the absence of acetate, but to be promoted by...
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| Published in | Biochimica et biophysica acta Vol. 124; no. 2; pp. 389 - 394 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
Elsevier B.V
24.08.1966
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0304-4165 0006-3002 1872-8006 |
| DOI | 10.1016/0304-4165(66)90202-9 |
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| Abstract | There are significant differences in the behaviour of oxy-, deoxy- and ferrihaemoglobin when studied under dissociating conditions by the Sephadex method.
The dissociation of oxyhaemoglobin appears to be insensitive to a change of pH from 7.0 to 5.5, in the absence of acetate, but to be promoted by high ionic strength. On the other hand, ferrihaemoglobin was found to be sensitive to both changes of pH and ionic strength, while deoxyhaemoglobin was not. |
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| AbstractList | There are significant differences in the behaviour of oxy-, deoxy- and ferrihaemoglobin when studied under dissociating conditions by the Sephadex method.
The dissociation of oxyhaemoglobin appears to be insensitive to a change of pH from 7.0 to 5.5, in the absence of acetate, but to be promoted by high ionic strength. On the other hand, ferrihaemoglobin was found to be sensitive to both changes of pH and ionic strength, while deoxyhaemoglobin was not. |
| Author | Merrett, T. |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/5968907$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1038/153406a0 10.1021/ja01852a005 10.1021/bi00893a021 10.1016/S0021-9258(18)64373-X 10.1016/S0021-9258(18)71190-3 |
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| References | Cunningham (BIB8) 1954; 211 Theorell, Ehrenberg (BIB6) 1955; Vol. 2 Rossi-Fanelli (BIB2) 1949 Theorell, Akesson (BIB10) 1941; 63 Svedberg, Pedersen (BIB9) 1940 Rossi-Fanelli, Antonini, Caputo (BIB1) 1961; 236 Edsall (BIB11) 1953; Vol. 1 Gutfreund (BIB5) 1944; 153 Bovey, Yanari (BIB7) 1960; Vol. 4 Ackers (BIB3) 1964; 3 Johnson (BIB4) 1948; Vol. 43 Rossi-Fanelli (10.1016/0304-4165(66)90202-9_BIB1) 1961; 236 Johnson (10.1016/0304-4165(66)90202-9_BIB4) 1948; Vol. 43 Gutfreund (10.1016/0304-4165(66)90202-9_BIB5) 1944; 153 Rossi-Fanelli (10.1016/0304-4165(66)90202-9_BIB2) 1949 Theorell (10.1016/0304-4165(66)90202-9_BIB6) 1955; Vol. 2 Bovey (10.1016/0304-4165(66)90202-9_BIB7) 1960; Vol. 4 Theorell (10.1016/0304-4165(66)90202-9_BIB10) 1941; 63 Ackers (10.1016/0304-4165(66)90202-9_BIB3) 1964; 3 Svedberg (10.1016/0304-4165(66)90202-9_BIB9) 1940 Edsall (10.1016/0304-4165(66)90202-9_BIB11) 1953; Vol. 1 Cunningham (10.1016/0304-4165(66)90202-9_BIB8) 1954; 211 |
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| SubjectTerms | Buffers Chromatography, Gel Hemoglobins Humans Hydrogen-Ion Concentration In Vitro Techniques Molecular Weight Phosphates Sodium Chloride Spectrophotometry |
| Title | Observations on the dissociation of oxy-, deoxy- and ferrihaemoglobin in relation to pH and ionic strength by the Sephadex method |
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