Observations on the dissociation of oxy-, deoxy- and ferrihaemoglobin in relation to pH and ionic strength by the Sephadex method

There are significant differences in the behaviour of oxy-, deoxy- and ferrihaemoglobin when studied under dissociating conditions by the Sephadex method. The dissociation of oxyhaemoglobin appears to be insensitive to a change of pH from 7.0 to 5.5, in the absence of acetate, but to be promoted by...

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Bibliographic Details
Published inBiochimica et biophysica acta Vol. 124; no. 2; pp. 389 - 394
Main Author Merrett, T.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 24.08.1966
Subjects
Buffers
Chromatography, Gel
Hemoglobins
Humans
Hydrogen-Ion Concentration
In Vitro Techniques
Molecular Weight
Phosphates
Sodium Chloride
Spectrophotometry
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Summary:There are significant differences in the behaviour of oxy-, deoxy- and ferrihaemoglobin when studied under dissociating conditions by the Sephadex method. The dissociation of oxyhaemoglobin appears to be insensitive to a change of pH from 7.0 to 5.5, in the absence of acetate, but to be promoted by high ionic strength. On the other hand, ferrihaemoglobin was found to be sensitive to both changes of pH and ionic strength, while deoxyhaemoglobin was not.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/0304-4165(66)90202-9