Ascaris lumbricoides: Extraglycolytic phosphorylation in intact muscle

• Published in: Experimental parasitology Vol. 23; no. 2; pp. 244 - 253
• Main Authors: Fischerová, Heda, Kubištová, Jarmila
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.1968
• Subjects: Adenine Nucleotides - metabolism; Adenosine Triphosphate - metabolism; Ascaris - metabolism; Glycolysis; Iodoacetates - pharmacology; Malates - pharmacology; Malonates - pharmacology; Muscles - metabolism; Oxidative Phosphorylation; Time Factors; NAD; NADH; AMP; x̄Δ; Δ ∼ P; ADP; ATP
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/0014-4894(68)90065-9

If isolated Ascaris lumbricoides muscle (preparations of one-half of the body wall) are kept in aerated del Castillo (1964) solution, their content of ATP, ADP, and AMP remains constant for at least 90 minutes. Addition of 7 × 10 −3 M iodoacetic acid brings about a rapid decomposition of ATP accompanied by an increase in ADP and AMP concentration. After addition of malate to the iodoacetate-poisoned muscle the ATP decrease is significantly slower. This malate effect is counteracted by malonate under anaerobic, but not under aerobic conditions. The results are in quantitative agreement with the assumption that malate metabolism accompanied by ATP synthesis occurs in Ascaris muscle: under aerobic conditions oxidation via NAD-dehydrogenase-flavine oxidase system, in anaerobiosis dismutation to succinate and (probably) pyruvate.