Patient-specific stem cell lines derived from human parthenogenetic blastocysts

Parthenogenetic activation of human oocytes may be one way to produce histocompatible cells for cell-based therapy. We report the successful derivation of six pluripotent human embryonic stem cell (hESC) lines from blastocysts of parthenogenetic origin. The parthenogenetic human embryonic stem cells...

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Published inCloning and stem cells Vol. 9; no. 3; p. 432
Main Authors Revazova, E S, Turovets, N A, Kochetkova, O D, Kindarova, L B, Kuzmichev, L N, Janus, J D, Pryzhkova, M V
Format Journal Article
LanguageEnglish
Published United States 01.09.2007
Subjects
Alkaline Phosphatase - metabolism
Blastocyst - cytology
Blastocyst - enzymology
Cell Line
Embryonic Stem Cells - cytology
Embryonic Stem Cells - enzymology
Genomic Imprinting
Germ Layers - cytology
HLA Antigens - metabolism
Humans
Karyotyping
Oocytes - cytology
Oocytes - enzymology
Parthenogenesis
Pluripotent Stem Cells - cytology
Pluripotent Stem Cells - enzymology
Telomerase - metabolism
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Summary:Parthenogenetic activation of human oocytes may be one way to produce histocompatible cells for cell-based therapy. We report the successful derivation of six pluripotent human embryonic stem cell (hESC) lines from blastocysts of parthenogenetic origin. The parthenogenetic human embryonic stem cells (phESC) demonstrate typical hESC morphology, express appropriate markers, and possess high levels of alkaline phosphatase and telomerase activity. The phESC lines have a normal 46, XX karyotype, except one cell line, and have been cultured from between 21 to 35 passages. The phESC lines form embryoid bodies in suspension culture and teratomas after injection to immunodeficient animals and give differentiated derivatives of all three embryonic germ layers. DNA profiling of all six phESC lines demonstrates that they are MHC matched with the oocyte donors. The study of imprinted genes demonstrated further evidence of the parthenogenetic origin of the phESC lines. Our research has resulted in a protocol for the production of human parthenogenetic embryos and the derivation of stem cell lines from them, which minimizes the presence of animal-derived components, making the derived phESC lines more suitable for potential clinical use.
ISSN:1536-2302
DOI:10.1089/clo.2007.0033