Comparative study of pyloric and fundic acid proteases in the guinea pig gastric mucosa: Evidence for a pyloric pepsinogen

1. 1. In the gastric mucosa of the guinea pig, fundic and pyloric extracts exhibit similar proteolytic activity and identical enzymatic properties. The Michaelis constant was denned at approximately 10 −4 M. 2. 2. The optimum of the reaction was demonstrated at pH 1.6 and 50°C. 3. 3. The activation...

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Published inComparative biochemistry and physiology. A, Comparative physiology Vol. 49; no. 2; pp. 241,IN3,247 - 246,IN3,249
Main Authors Cheret, A.M, Soumarmon, A, Lewin, M, Amar-Costesec, A, Bonfils, S
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.10.1974
Subjects
Animals
Calorimetry
Cavia porcellus
Drug Stability
Electrophoresis
Enzyme Activation
gastric mucosa
Gastric Mucosa - enzymology
Guinea Pigs
Hot Temperature
Hydrogen-Ion Concentration
Kinetics
Organ Specificity
Pepsinogen
Pepsinogens - metabolism
Peptide Hydrolases - metabolism
Polysaccharides
pylorus
Pylorus - enzymology
Stomach - enzymology
Thermodynamics
gastric mucosa
Cavia porcellus
Pepsinogen
pylorus
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Summary:1. 1. In the gastric mucosa of the guinea pig, fundic and pyloric extracts exhibit similar proteolytic activity and identical enzymatic properties. The Michaelis constant was denned at approximately 10 −4 M. 2. 2. The optimum of the reaction was demonstrated at pH 1.6 and 50°C. 3. 3. The activation energy was 10 kcal/mole of substrate. 4. 4. Heat inactivation curves did not differ for pyloric and fundic extracts. 5. 5. Electrophoretic patterns were the same for both extracts.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0300-9629
DOI:10.1016/0300-9629(74)90113-3