Quantitative determination of pectic substances as an example of a rhamnopolysaccharide assay

• Published in: Journal of biochemical and biophysical methods Vol. 19; no. 2; pp. 215 - 222
• Main Authors: Turecek, P.L., Buxbaum, E., Pittner, F.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.1989
• Subjects: Carbohydrate Conformation; Carbohydrate Dehydrogenases; Kinetics; Molecular Sequence Data; NAD - analysis; Pectic substance; Pectin, assay of; Pectinase; Pectins - analysis; Polysaccharides - analysis; Rhamnopolysaccharide; Rhamnose - analysis; Rhamnose dehydrogenase, L; Rhamnose, L; Spectrophotometry, Ultraviolet - methods; Rhamnose, L; Rhamnose dehydrogenase, L; Rhamnopolysaccharide; Pectic substance; Pectin, assay of; Pectinase

A quantitative enzymatic assay for rhamnopolysaccharides is described. The procedure is shown with pectic substances as an example. The test is based on the enzymatic degradation of the macromolecules to liberate L-rhamnose. This sugar can be quantitatively determined with the help of L-rhamnose deh...