Quantitative determination of pectic substances as an example of a rhamnopolysaccharide assay

A quantitative enzymatic assay for rhamnopolysaccharides is described. The procedure is shown with pectic substances as an example. The test is based on the enzymatic degradation of the macromolecules to liberate L-rhamnose. This sugar can be quantitatively determined with the help of L-rhamnose deh...

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Bibliographic Details
Published inJournal of biochemical and biophysical methods Vol. 19; no. 2; pp. 215 - 222
Main Authors Turecek, P.L., Buxbaum, E., Pittner, F.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.08.1989
Subjects
Carbohydrate Conformation
Carbohydrate Dehydrogenases
Kinetics
Molecular Sequence Data
NAD - analysis
Pectic substance
Pectin, assay of
Pectinase
Pectins - analysis
Polysaccharides - analysis
Rhamnopolysaccharide
Rhamnose - analysis
Rhamnose dehydrogenase, L
Rhamnose, L
Spectrophotometry, Ultraviolet - methods
Rhamnose, L
Rhamnose dehydrogenase, L
Rhamnopolysaccharide
Pectic substance
Pectin, assay of
Pectinase
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Summary:A quantitative enzymatic assay for rhamnopolysaccharides is described. The procedure is shown with pectic substances as an example. The test is based on the enzymatic degradation of the macromolecules to liberate L-rhamnose. This sugar can be quantitatively determined with the help of L-rhamnose dehydrogenase under concomitant reduction of NAD, thus allowing the quantitative evaluation of the original pectin.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0165-022X
1872-857X
DOI:10.1016/0165-022X(89)90028-6