Human cysteine dioxygenase type I: primary structure derived from base sequencing of cDNA

• Published in: Biochimica et biophysica acta Vol. 1209; no. 1; pp. 107 - 110
• Main Authors: McCann, Karen P., Akbari, Mohammed T., Williams, Adrian C., Ramsden, David B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 16.11.1994
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; Cysteine Dioxygenase; Dioxygenases; DNA Probes; DNA, Complementary; Gene Library; Human; Humans; Liver; Liver - enzymology; Molecular Sequence Data; Oxygenases - genetics; Polymerase Chain Reaction - methods; Rats; RNA, Messenger - genetics; RNA-Directed DNA Polymerase; Sequence Homology, Amino Acid; Tumor Cells, Cultured; Human; Cysteine dioxygenase; Liver
• ISSN: 0167-4838; 0006-3002; 1879-2588
• DOI: 10.1016/0167-4838(94)90144-9

The base sequence of cDNA encoding the complete human liver cysteine dioxygenase type I (CDO-I; EC 1.13.11.20) message, and the derived amino-acid sequence are reported. CDO-I is encoded on a single mRNA species (approx. 1.5 kb). Human CDO-I clones were identified by screening a liver cDNA library, and inserts were isolated and sequenced. In addition, human liver total RNA was reverse-transcribed and CDO-I cDNA amplified by PCR using a modified poly-T primer and specific CDO-I primers to give a second source of sequencing template. The CDO-I message encodes a 200 amino-acid residue protein, the sequence of which has greater than 90% homology with the equivalent rat enzyme. The message was not expressed in a human hepatoma cell line (Hep G2).