Features of disruption mutants of genes encoding for hydrophobin Vmh2 and Vmh3 in mycelial formation and resistance to environmental stress in Pleurotus ostreatus

• Published in: FEMS microbiology letters Vol. 370
• Main Authors: Han, Junxian, Kawauchi, Moriyuki, Schiphof, Kim, Terauchi, Yuki, Yoshimi, Akira, Tanaka, Chihiro, Nakazawa, Takehito, Honda, Yoichi
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 17.01.2023
• Subjects: Air-water interface; Environmental stress; Fungal Proteins - metabolism; Genes; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Hydrophobicity; Hydrophobins; Hyphae; Hyphae - genetics; Microbiology; Mycelia; Mycelium - genetics; Mycelium - metabolism; Pleurotus - genetics; Pleurotus - metabolism; Pleurotus ostreatus; Transmission electron microscopy; Water - chemistry; Water drops; hydrophobin; Vmh; environmental stress; cell wall; Agaricomycete; Pleurotus ostreatus
• ISSN: 1574-6968; 0378-1097; 1574-6968
• DOI: 10.1093/femsle/fnad036

Abstract Hydrophobins, which are small-secreted proteins with both hydrophobic and hydrophilic parts, can self-assemble into an amphiphilic film at the air-water interface, helping the fungus to form aerial hyphae. In the agaricomycete Pleurotus ostreatus, more than 20 putative hydrophobin genes have been predicted. Of these, two hydrophobin genes, vmh2 and vmh3, are predominantly expressed in the vegetative mycelium. In this study, we focused on the functions of Vmh2 and Vmh3 in vegetative mycelia. Based on the observation of the mycelial cross-section by transmission electron microscopy and the disappearance time of water droplets on the mycelial surface, Vmh2 and Vmh3 were considered essential for the maintenance of the surface hydrophobicity of the mycelium. The Δvmh3 and Δvmh2Δvmh3 strains exhibited relatively slower aerial mycelia formation on a liquid medium, and no significant alteration was observed in Δvmh2 strains. Only the Δvmh3 and Δvmh2Δvmh3 strains grew slower than the wild-type strain under stress conditions involving SDS and H2O2 on agar plates. This study revealed possible distinct roles for these hydrophobins in stress resistance. These results suggest that Agaricomycetes, including P. ostreatus, have evolved to possess multiple different hydrophobins as a means of adapting to various environments. The first report of functional differences between hydrophobins Vmh2 and Vmh3 in Pleurotus ostreatus.