A monoclonal antibody to an interspecies major histocompatibility determinant inhibits a virus-specific T-cell clone

• Published in: Cellular immunology Vol. 68; no. 1; pp. 193 - 198
• Main Authors: Allouche, Michele, Bennink, Jack R., McKearn, Thomas J., Doherty, Peter C.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 15.03.1982
• Subjects: Animals; Antibodies, Monoclonal; Binding, Competitive; Clone Cells - immunology; Cytotoxicity, Immunologic; Epitopes; H-2 Antigens - immunology; Histocompatibility Antigens - immunology; In Vitro Techniques; Influenza A virus - immunology; Mice; Mice, Inbred BALB C; Rats; Rats, Inbred Strains; Species Specificity; T-Lymphocytes - immunology
• ISSN: 0008-8749; 1090-2163
• DOI: 10.1016/0008-8749(82)90103-4

The cytotoxicity of an influenza-specific T-cell clone of BALB/c mouse origin for an H-2 compatible, virus-infected target is greatly inhibited by a monoclonal antibody which binds to mouse major histocompatibility complex (MHC) determinants, but was originally prepared against MHC antigens of the Brown Norway (BN) rat. The inhibition is still observed after absorption of the antibody with lymphoid cells from Lewis, but not from BN or Lewis. l N rats. It thus seems that the site blocked by this monoclonal antibody, which interacts with MHC antigens from a number of animal species, is at least close to a determinant on the MHC glycoprotein that is involved in T-cell recognition. This reagent may be useful for comparative analysis of the phylogeny of MHC-restricted T-cell responses in different species.