Gas chromatographic determination of urinary 3-methoxy-4-hydroxyphenylethyleneglycol sulphate after its ion-pair extraction

In order to develop a gas Chromatographic method with flame ionization detection for the analysis of urinary 3-methoxy-4-hydroxyphenylethyleneglycol sulphate, a new procedure for its isolation has been introduced. The conjugated metabolite has been extracted by an ion-pair technique using tetrabutyl...

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Bibliographic Details
Published inClinica chimica acta Vol. 121; no. 1; pp. 79 - 86
Main Authors Biondi, Pier Antonio, Niu-Fan, Gu, Secchi, Camillo
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 06.05.1982
Subjects
Adult
Child
Child, Preschool
Chromatography, Gas - methods
Detergents
Female
Glycols - urine
Humans
Male
Methoxyhydroxyphenylglycol - isolation & purification
Methoxyhydroxyphenylglycol - urine
Methylene Chloride
Neuroblastoma - urine
Quaternary Ammonium Compounds
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Summary:In order to develop a gas Chromatographic method with flame ionization detection for the analysis of urinary 3-methoxy-4-hydroxyphenylethyleneglycol sulphate, a new procedure for its isolation has been introduced. The conjugated metabolite has been extracted by an ion-pair technique using tetrabutyl ammonium as counter ion and dichloromethane as organic solvent. Hydrolysis and derivatization of 3-methoxy -4-hydroxyphenylethyleneglycol sulphate have been finally performed by treatment with heptafluorobutyric anhydride. Each step of the procedure, applied to the analysis of urine samples both from normal subjects and from patients with neuroblastoma, has been discussed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-8981
1873-3492
DOI:10.1016/0009-8981(82)90213-3