The requirement of mammalian cell cultures for serum proteins. Growth-promoting activity of pepsin-digested serum albumin in different media

Both serum albumin and a human serum globulin fraction (containing mainly α- and β-globulins) were required for continued cell reproduction in vitro of a murine mastocytoma characterized by lack of cell attachment to glass surfaces. The hydrolysate prepared by digestion of bovine serum albumin with...

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Published inBiochimica et biophysica acta Vol. 147; no. 1; pp. 154 - 161
Main Authors Schaer, J.C., Schindler, R.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 19.09.1967
Subjects
Animals
Cattle
Cell Division
Culture Media
Culture Techniques
Growth Substances
Humans
Neoplasms, Experimental
Pepsin A
Protein Hydrolysates
Serum Albumin, Bovine
Serum Globulins
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Summary:Both serum albumin and a human serum globulin fraction (containing mainly α- and β-globulins) were required for continued cell reproduction in vitro of a murine mastocytoma characterized by lack of cell attachment to glass surfaces. The hydrolysate prepared by digestion of bovine serum albumin with pepsin exhibited part of the growth-promoting activity of the intact protein. A mixture of several chemically defined substances (7 ‘non-essential’ amino acids, pyruvate, thymidine, hypoxanthine, folinic acid, vitamin B 12, spermidine, linoleic acid, lipoic acid, insulin, and methylcellulose), when added to a minimal medium in place of pepsin-degraded bovine serum albumin, had the same effect on cell multiplication as the bovine serum albumin hydrolysate. Bovine serum albumin hydrolysate as well as the chemically defined medium supplements, however, did not support continued cell reproduction.
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ISSN:0005-2795
0006-3002
1879-2952
DOI:10.1016/0005-2795(67)90098-0