A fluorometric method for the enzymatic determination of normal concentrations of urinary glucose
An enzymatic fluorometric method is described for the determination of the low glucose concentrations normally present in the urine from subjects in the fasting state. The method is an adaptation of existing spectrophotometric procedures using hexokinase (HK) and glucose-6-phosphate dehydrogenase (G...
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| Published in | Clinica chimica acta Vol. 18; no. 3; pp. 383 - 393 |
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| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
Elsevier B.V
01.12.1967
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0009-8981 1873-3492 |
| DOI | 10.1016/0009-8981(67)90035-6 |
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| Abstract | An enzymatic fluorometric method is described for the determination of the low glucose concentrations normally present in the urine from subjects in the fasting state. The method is an adaptation of existing spectrophotometric procedures using hexokinase (HK) and glucose-6-phosphate dehydrogenase (G-6-PDH). The non-specific fluorescence of urine is almost completely removed by treating the urine with a mixed ion exchange resin. The chief advantage of this method over the spectro-photometric procedure is its low cost. |
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| AbstractList | An enzymatic fluorometric method is described for the determination of the low glucose concentrations normally present in the urine from subjects in the fasting state. The method is an adaptation of existing spectrophotometric procedures using hexokinase (HK) and glucose-6-phosphate dehydrogenase (G-6-PDH). The non-specific fluorescence of urine is almost completely removed by treating the urine with a mixed ion exchange resin. The chief advantage of this method over the spectro-photometric procedure is its low cost. |
| Author | Scherstén, Bengt Tibbling, Gunnar |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/6081879$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1007/BF01506150 10.1093/clinchem/4.6.462 10.2337/diab.5.1.1 10.1093/clinchem/11.3.367 10.1016/S0021-9258(18)56141-X 10.1093/clinchem/6.5.466 10.1001/jama.201.12.949 10.1016/S0021-9258(18)64996-8 10.1085/jgp.29.6.379 10.1007/BF01478633 10.2337/diab.15.2.127 10.1093/ajcp/32.2_ts.195 10.1136/bmj.1.5444.1209 10.3109/00365516009065404 10.1093/clinchem/7.4.289 10.1021/ac60147a040 10.1016/S0021-9258(18)56270-0 10.1093/clinchem/11.4.471 |
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| SubjectTerms | Buffers Chemistry, Clinical Fluorometry Glucosephosphate Dehydrogenase Glycosuria Humans Hydrogen-Ion Concentration Ion Exchange Resins Kinetics Methods Statistics as Topic |
| Title | A fluorometric method for the enzymatic determination of normal concentrations of urinary glucose |
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