A fluorometric method for the enzymatic determination of normal concentrations of urinary glucose

An enzymatic fluorometric method is described for the determination of the low glucose concentrations normally present in the urine from subjects in the fasting state. The method is an adaptation of existing spectrophotometric procedures using hexokinase (HK) and glucose-6-phosphate dehydrogenase (G...

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Published inClinica chimica acta Vol. 18; no. 3; pp. 383 - 393
Main Authors Scherstén, Bengt, Tibbling, Gunnar
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.12.1967
Subjects
Buffers
Chemistry, Clinical
Fluorometry
Glucosephosphate Dehydrogenase
Glycosuria
Humans
Hydrogen-Ion Concentration
Ion Exchange Resins
Kinetics
Methods
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ISSN0009-8981
1873-3492
DOI10.1016/0009-8981(67)90035-6

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Summary:An enzymatic fluorometric method is described for the determination of the low glucose concentrations normally present in the urine from subjects in the fasting state. The method is an adaptation of existing spectrophotometric procedures using hexokinase (HK) and glucose-6-phosphate dehydrogenase (G-6-PDH). The non-specific fluorescence of urine is almost completely removed by treating the urine with a mixed ion exchange resin. The chief advantage of this method over the spectro-photometric procedure is its low cost.
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ISSN:0009-8981
1873-3492
DOI:10.1016/0009-8981(67)90035-6