Clastogenic activity of bile acids and organic acid fractions of human feces

Chloroform extracts of fecal material from 4 subjects on normal mixed western diets were fractionated to obtain an acid fraction and a hexane extract containing neutrals and bases. The acid fraction from at least 2 of the donors induced an elevated frequency of chromosomal aberrations and exchanges...

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Bibliographic Details
Published inCancer letters Vol. 15; no. 3; pp. 317 - 327
Main Authors Barnes, William S., Powrie, William D.
Format Journal Article
LanguageEnglish
Published Ireland Elsevier Ireland Ltd 01.03.1982
Subjects
Acids - analysis
Animals
Bile Acids and Salts - adverse effects
Bile Acids and Salts - analysis
Bile Acids and Salts - metabolism
Chemical Fractionation
Chromosome Aberrations
Cricetinae
Feces - analysis
Humans
Methanol - adverse effects
Methanol - analysis
Mutagenicity Tests
Ursodeoxycholic Acid - adverse effects
Ursodeoxycholic Acid - metabolism
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Summary:Chloroform extracts of fecal material from 4 subjects on normal mixed western diets were fractionated to obtain an acid fraction and a hexane extract containing neutrals and bases. The acid fraction from at least 2 of the donors induced an elevated frequency of chromosomal aberrations and exchanges in Chinese hamster ovary (CHO) cells. Since acid steroids are expected to be present in the acid fraction, 5 bile acids were assayed for clastogenic activity in CHO cells. Ursodeoxycholic acid induced chromosomal aberrations and exchanges, and this effect was enhanced by the addition of a microsomal S9 mix. However, the enhancement is probably due to physical factors rather than to enzymatic activity.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0304-3835
1872-7980
DOI:10.1016/0304-3835(82)90133-1